Microsomes from the PaCPT/PaCBP-expressing yeast efficiently incorporated 14C-isopentenyl diphosphate into dehydrodolichyl diphosphates however, NR with high molecular weight could not be synthesized in in vitro assays. Co-expression of both more » PaCBP and each of the PaCPTs could complemented the dolichol (a short cis-polyisoprene)-deficient yeast, whereas the individual expressions could not. In this work, we identified four guayule genes - three PaCPTs ( PaCPT1-3) and one PaCBP, whose protein products organize PaCPT/PaCBP complexes. Recently, implications of the protein complex involving cis-prenyltransferases (CPTs) and CPT-Binding Proteins (CBPs) in NR biosynthesis were shown in lettuce and dandelion, but such protein complexes have yet to be examined in guayule. Despite its potential to be an alternative NR supplier, the enzymes for cis-polyisoprene biosynthesis have not been comprehensively studied in guayule. Guayule ( Parthenium argentatum) is a perennial shrub in the Asteraceae family and synthesizes a high quality, hypoallergenic cis-1,4-polyisoprene (or natural rubber NR). National Energy Research Scientific Computing Center (NERSC) Sponsoring Org.: USDOE Office of Science (SC) National Science Foundation (NSF) California Institute for Mexico and the United States Consejo Nacional de Ciencia y Tecnologia de Mexico OSTI Identifier: 1816791 Grant/Contract Number: AC02-05CH11231 DEB-1011606 1519732 Resource Type: Journal Article: Accepted Manuscript Journal Name: Proceedings of the National Academy of Sciences of the United States of America Additional Journal Information: Journal Volume: 117 Journal Issue: 52 Journal ID: ISSN 0027-8424 Publisher: National Academy of Sciences Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES adaptation gene flow hybrid zone reciprocal transplant = concentration. Publication Date: Mon Dec 14 00:00: Research Org.: Lawrence Berkeley National Lab. of California, Davis, CA (United States).
of California, Los Angeles, CA (United States). School of the Environment Florida International Univ., Miami, FL (United States).
After finding evidence for extremely strong selection at both hybrid zones, we use a combination of field experiments, high-resolution imaging, and physiological measurements to determine the ecological basis for selection at one of the hybrid zones. For a recent radiation of New World desert shrubs ( Encelia: Asteraceae), we use fine-scale geographic sampling and population genomics to determine patterns of gene flow across two hybrid zones formed between two independent pairs of species with parapatric distributions. For species in which potential gene flow is high but realized gene flow is low, adaptation via natural selection may be a particularly important force maintaining species.
Sympatric speciation and ecotone driver#
Natural selection is an important driver of genetic and phenotypic differentiation between species.
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